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Image Search Results
Journal: Biology Open
Article Title: Site-1 protease ablation in the osterix-lineage in mice results in bone marrow neutrophilia and hematopoietic stem cell alterations
doi: 10.1242/bio.052993
Figure Lengend Snippet: Stefin protein selectively inhibits osteogenesis. (A) In vitro osteogenic differentiation assays with BMSCs from P21 WT (S1P f/f ) mice with or without (CTRL) His6-Stfa1 or His6-Stfa2 proteins at 250 ng/ml. Typical results are shown from N =4 at day 14. Assays were done in duplicate with upper panels stained for von Kossa and lower panels with Hematoxylin. (B) qPCR analysis at day 7 for Pro-collagen1 (procollagen type I), Alp (alkaline phosphatase) and Bglap (osteocalcin) with RNA harvested from WT BMSCs treated with 250 ng/ml of His6-Stfa1 or His6-Stfa2 (mean±s.d.; N =4-5; ** P =0.0079). (C) Immunocytochemistry for His6-tag at day 3 on BMSCs treated with 250 ng/ml of His6-Stfa1 or His6-Stfa2 (Red=His6 tag; Blue=DAPI-stained nuclei). Scale bar: 50 µm. (D) In vitro adipogenic differentiation assays with BMSCs from P21 WT (S1P f/f ) mice with or without (CTRL) His6-Stfa1 or His6-Stfa2 proteins at 500 ng/ml. Typical results are shown from N =4 at day 7.
Article Snippet: The cells were then cultured in two wells of a six-well plate, grown until confluent at which point osteogenic differentiation media (α-MEM with 10% FBS, 2% penicillin/streptomycin, 10 mM β-glycerophosphate, 50 μg/ml of ascorbic acid), or
Techniques: In Vitro, Staining, Immunocytochemistry